Monoclonal antibodies (mAbs) have proven to be very versatile agents for the treatment of diseases including cancer and inflammatory diseases. The discovery and screening of antibodies with high affinity for antigens plays an integral and important role in drug discovery and immunology. Antibody display technology is an important method for the successful isolation of antigen-specific antibodies.
Figure.1 Schematic structure of IgG/mAb. (VH, variable structural domain of heavy chain; VL, variable region of light chain; CL, constant structural domain of light chain; CH1-CH3, constant region of heavy chain; H, hinge region; Fab, antigen-binding fragment; Fc, crystallizable fragment.)
Antibody display technology adheres to the principle of genotype-phenotype coupling, enabling the isolation of key features of the desired drug candidate based on the association of the protein of interest (phenotype) with its genetic information (genotype). The principle of genotype-phenotype coupling allows the coding of up to several billion different protein variants, the binding of which can be selected in an iterative process through high-throughput identification. Phage display technology is the most established of the antibody display technologies. High-throughput sequencing-based antibody library sequencing enables the detection of the original synthetic antibody library antibody gene composition and respective abundance for pre-experimental QC work. After antigen screening, antibody library sequencing enables the discovery of antibody sequences that bind with high affinity to the antigen and are used for downstream optimization of synthetic expression.
CD Genomics has introduced NGS into the phage display workflow, which not only greatly improves the efficiency of specific antibody screening, but also provides deeper and more comprehensive coverage. Our NGS-based technology platform breaks down sequencing limitations to provide lower cost and higher quality data. Our antibody display sequencing can detect antibody library antibody gene composition and respective abundance for pre-experimental QC work. Following antigen screening, antibody library sequencing can be used to discover antibody sequences that bind with high affinity to the antigen and can be used downstream to optimize synthetic expression.
1. Sequencing sample requirements
| Sample Type | RNA Amount | RNA Concentration | Purity |
|---|---|---|---|
| Purified phage, hybrid phage vector DNA and amplicons containing variable regions of antibody light and heavy chains | ≥ 1 μg | ≥ 100 ng/μL | OD260/280=1.8 - 2.0; no degradation, no contamination |
2. Library construction and NGS
NGS-based Illumina / MGI platform
3. Bioinformatics Analysis
CD Genomics' antibody display sequencing service will greatly enhance the screening process for antigen affinity antibodies. We provide researchers with higher throughput methods for antibody discovery, screening, and application. These applications include antibody-based drug design, screening and detection of target antigens, and other biomedical research.
CD Genomics is committed to combining professional project protocol guidance and standardized analysis processes to ensure that your project is performed accurately and quickly. If you are interested in CD Genomics' antibody display sequencing service in the field of antibody discovery, please feel free to contact us now.
CD Genomics is transforming biomedical potential into precision insights through seamless sequencing and advanced bioinformatics.
