TILLING ( Targeting Induced Local Lesions IN Genomes ), a novel approach to reverse genetics research, uses traditional chemical mutagenesis ( EMS ) to create libraries of mutagenized individuals that are subsequently screened for mutations using high-throughput assays to rapidly and efficiently identify point mutations from mutagenized populations. TILLING has been applied to the study of a variety of organisms.

CD Genomics offers a mutation screening method based on advanced sequencing technology that sequences target genes amplified from a multidimensional pool of templates representing several hundred individuals per experiment. We optimize the TILLING service process, helps our clients save significant time and effort and greatly accelerate research in the agricultural field.

Technical advantages of TILLING

The generation of TILLING overcomes the limitations of gene knockout method and has unique advantages.

• Relatively simple technique.
• Predict mutation frequency and obtain satisfactory mutation density.
• High throughput, automated operation, rapid identification results.

TILLING accelerates research and practice in the field of agriculture

Our TILLING service, which relies on traditional chemical mutagenesis, is easily adapted to most crops. The rest of the various mutation discovery methods can also be effectively used for TILLING screening, our TILLING technology has shown important application value in functional genomics research and crop genetics breeding, by identifying a large number of target mutants containing different or new, etc. The combination of allelic variation and conventional breeding technology can realize gene aggregation of excellent allelic variation of target traits, create new breeding materials with excellent comprehensive traits, and cultivate new crop varieties with stress tolerance, high yield and high quality.

• Analyzing Gene Functions
• Mining important genetic resources
• Improving crop quality
• Breeding superior varieties

So far, our TILLING technology has been successfully applied to dozens of species of plants and animals. As our TILLING service continues to expand, more and more species of organisms have been effectively studied and applied using the TILLING technology, and the important application value of TILLING in plant and animal research has become more and more evident.

TILLING procedure

Step 1. We develop a mutagenic population. The chemical mutagen ethyl methanesulfonate (EMS) is usually used. The goal is to obtain a high density of induced point mutations while maintaining appropriate viability and fecundity.

Step 2. We screen the DNA library for mutagenesis induction. By classical mismatch cutting and fluorescence detection, 30 point allelic mutations can be identified within 1 week using a single DNA analyzer.

Final step. We discover the specific effects on the mutant plants by testing the mutants found.

Fig. 2. Overview of the TILLING procedure. (Till BJ, et al., 2018)

Our advantages and features

• Flexible and customized experimental solution.
• Advanced experimental platform and excellent technical team of experts.
• Considerate after-sales services.
• Comprehensive bioinformatics analysis services.

CD Genomics has advanced sequencing platforms and rich experience in project services, a standardized service model, and a strict quality control system to deliver accurate and reliable data results, as well as to meet your various needs in project research. We are committed to providing high quality, cost-effective sequencing services to better support your scientific research.If you are interested in us, please feel free to contact us.

Reference

1. Till BJ, et al. (2018). "TILLING: The Next Generation." Nat Biotechnol. 164:139-160.