Multiplex SNaPshot, a typing technique based on the principle of fluorescently labelled single base extensions, is aimed at medium throughput SNP typing projects. The main principle is the single base extension technique, by designing primers in the immediate vicinity of the SNP site, adding SNP site primers to the reaction, amplifying the PCR product with SNP sites with fluorescently labelled ddNTP, the primers take on the fluorescence associated with the bases of the SNP sites after the single base extension, and the reaction product is analysed on a sequencer to obtain information on the SNP sites, usually the The reaction products are analysed on the sequencer to obtain information about the SNP site, usually one reaction is able to detect multiple SNP sites.

CD Genomics offers advanced snapshot genotyping methods that are highly sensitive, reproducible and require no additional equipment, and have been widely used for the detection of SNPs in plant and animal genes. We have extensive experience in helping researchers to screen and identify SNPs in plant and animal research, and in detecting small sequence variants in plant and animal genomes. Our snapshot-based SNP markers have been invaluable in advancing plant and animal genetics research.

Technical features

• High precision. Accuracy up to Sanger sequencing level.
• Cost effective. High efficiency with low cost of ownership.
• More sensitive allele detection. Rare alleles can be detected.
• Simultaneous detection of multiple loci. Multiple SNPs can be interrogated simultaneously in a single reaction.
• Not limited by SNP locus polymorphism.
• Not limited by sample size.

Our services can be applied to the following research areas

• Population genetic diversity analysis
• Genetic breeding of plants and animals
• Molecular marker development
• Genotyping of plants and animals

Instructions for providing samples

Service workflow

Fig 2. Multiplex SNaPshot workflow

Our advantages and features

• Advanced experimental platform. Enables high-throughput, multi-locus detection.
• High efficiency. Our services are characterised by high detection rates and short cycle times.
• Accurate and reliable results. We promise to send you a full report and guarantee the accuracy of the results.
• After-sales service. We provide a comprehensive after-sales service to reduce your worries.

CD Genomics has extensive experience in plant and animal genotyping research and we have successfully genotyped more than a dozen species of plants and animals. We provide high quality genotyping services to provide reliable and accurate results to help researchers in genetic mapping and molecular marker-assisted breeding in plant and animal research. If you are interested in our services, please feel free to contact us.