Marker-assisted selection (MAS) is the process of selecting for agriculturally important traits (e.g., productivity, disease resistance, abiotic traits) in crop breeding using morphological, biochemical, or DNA markers as indirect selection criteria. The approach involves the selection of plants carrying genomic regions involved in the expression of traits of interest through the application of molecular markers to improve the efficiency of plant breeding through the precise transfer of genomic regions of interest and accelerated recovery of the genome of the rotating parent. The development and availability of a range of molecular markers and dense molecular genetic maps in crops have enabled the application of MAS. The success of MAS depends on several factors, including the number of target genes to be transferred and the distance between the flanking markers and the target genes. The importance of MAS as a tool for crop improvement has been extensively studied in different crop species and for different traits.
Fig. 1. MAS for rice stress tolerance improvement. (Das et al., 2017)
Our MAS solution
CD Genomics has deep theoretical knowledge of genomics and offers a range of advanced technical services related to agricultural genomics. Our technical services play an important role in molecular plant breeding. Quantitative Trait Locus (QTL) mapping is the basis for MAS marker development. We provide QTL service to identify DNA markers, genes, and QTL associated with specific traits. In addition, Our NGS (next-generation sequencing) technology allows for the rapid and rational detection of SNPs within genes and serves as an intelligent tool for MAS in breeding programs.
CD Genomics is committed to developing a complete MAS solution to meet the diverse needs of plant breeders and researchers. Our MAS solutions are based on cutting-edge marker technologies, high-throughput genotyping, and association mapping populations. These services provide researchers with powerful tools to accelerate the breeding process, especially in enhancing stress tolerance traits in plants.
Workflow of MAS
(1) Population development: parental selection and hybridization.
(2) QTL mapping: linkage map construction phenotypic evaluation for trait OTL analysis.
(3) QTL validation: confirmation of position and effect of QTLs verification of QTLs in independent populations and testing in different genetic backgrounds fine mapping.
(4) Marker validation: testing of markers in important breeding material identification of 'toolbox' of polymorphic markers.
(5) Marker-assisted selection.
Advantages of MAS
Simpler than phenotypic screening, saving time, resources, and effort.
Can be performed at the seedling stage and can quickly eliminate unwanted plant genotypes.
Individual plants can be selected on the basis of genotype. For most traits, pure and heterozygous plants cannot be distinguished by conventional phenotypic screening.
The total number of lines to be tested can be reduced.
Applications of MAS in plant breeding
Marker-assisted evaluation of breeding material, e.g., varietal identification, genetic diversity assessment, parent selection, and hybrid confirmation.
Marker-assisted backcrossing
Marker-assisted pyramiding.
Early marker-assisted selection.
Combinatorial marker-assisted selection.
Our strengths and features
Cutting-edge technology: We utilize the latest marker technology to ensure the highest quality results for our clients.
Customized solutions: Our MAS services are tailored to meet the unique needs of each research or breeding project.
Accuracy and efficiency: Our expertise improves the accuracy and efficiency of plant breeding, especially for stress tolerance traits.
With years of experience and state-of-the-art services, CD Genomics is a trusted partner for researchers and breeders looking to harness the power of MAS. Our genome sequencing projects for rice and other crop species will provide a wealth of data that can be used for QTL mapping and marker development in other cereals. If you are interested, please feel free to contact us.
Reference
Das, Gitishree, Jayanta Kumar Patra, and Kwang-Hyun Baek. "Insight into MAS: a molecular tool for development of stress resistant and quality of rice through gene stacking." Frontiers in plant science 8 (2017): 985.
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OUR MISSION
CD Genomics is propelling the future of agriculture by employing cutting-edge sequencing and genotyping technologies to predict and enhance multiple complex polygenic traits within breeding populations.
Fig. 1. MAS for rice stress tolerance improvement. (Das et al., 2017)
