Epigenetic Sequencing

Epigenetic Sequencing

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Epigenetic sequencing

Epigenetics is a technique used to study heritable changes in gene expression and regulation based on high-throughput sequencing. Epigenetic modifications play a crucial role in gene expression, affecting normal cellular development, regulation and maintenance of the organism. In addition, the environment in which plants and animals live and the nutrients they draw from may also lead to epigenetic changes when they are altered.

CD Genomics provides epigenetic sequencing services based on NGS high-throughput sequencing foundation, which requires lower starting sample size, lower cost, shorter time, and can reduce a large number of unnecessary redundant genes, improving the efficiency of research in specific target regions. Different epigenetic modifications lead to different changes in living organisms. Our epigenetic sequencing service helps customers to study epigenetic modifications at the genome level, to explore the mechanism of dynamic network formation of life regulation, and to explore heritable changes in gene expression, regulation and traits with no change in nucleic acid sequence. The common epigenetic sequencing methods we provide based on NGS technology are whole genome hydroxymethylation sequencing, ChIP-seq, RIP-seq, and MeRIP-seq.

Applications of our epigenetic sequencing in animal and plant research

Genome-wide hydroxymethylation sequencing in plant and animal research

  • Plant and animal cell differentiation
  • Plant and animal tissue development
  • Plant and animal breeding

RIP-seq in plant and animal research

  • Validation of RNA-target protein interactions and precise binding sites.
  • Genome-wide identification of RNA-RBP interaction networks.
  • Identification and functional mechanisms of non-coding RNAs in plants and animals.

We have successfully performed RIP-seq on dozens of species including rice, Arabidopsis, pig, chicken, sheep and others.

Applications of MeRIP-seq in plant and animal research

  • Plant and animal cell differentiation
  • Plant and animal development
  • Studying plant-virus interactions
  • Improving plant growth
  • Improving crop yields

We have completed MeRIP-seq on fish, cattle, pigs, chickens, rice, cabbage, pears and dozens of other species.

Epigenetic sequencing technology advantages

  • High accuracy
  • High specificity
  • Wide range of applications

Instructions for providing samples

Genome-wide hydroxymethylation sequencing RIP-seq MeRIP-seq
Cell sample volume > 5×106
Tissue sample volume > 100 mg
gDNA > 1 μg
Cell sample volume > 5×108
Tissue sample volume > 500 mg
RNA > 1 μg
Cell sample volume > 5×106
Tissue sample volume > 20 mg
RNA > 500 ng
Samples were placed in 1.5 mL EP tubes, sealed with sealing film and transported on dry ice or ice packs to avoid repeated freezing and thawing.

Sequencing workflow

Epigenetic sequencing workflow- CD GenomicsFig 2. Epigenetic sequencing workflow

Features of our services

  • Experienced service.
  • Comprehensive guidance, including experimental design and validation.
  • Advanced experimental platforms to efficiently develop and execute optimal experimental routes.
  • After-sales technical support.

CD Genomics has successfully sequenced a wide range of plant and animal epigenetics, and we provide customised experimental protocols and integrated bioinformatics analysis services to assist our clients with the analysis of large volumes of NGS-based data according to their research needs. Our scientific protocol design, strict standardised service procedures and professional technical team are committed to providing you with the highest quality project services. If you are interested in us, please feel free to contact us.

For Research Use Only.
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CD Genomics is propelling the future of agriculture by employing cutting-edge sequencing and genotyping technologies to predict and enhance multiple complex polygenic traits within breeding populations.

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