Epigenetics is a technique used to study heritable changes in gene expression and regulation based on high-throughput sequencing. Epigenetic modifications play a crucial role in gene expression, affecting normal cellular development, regulation and maintenance of the organism. In addition, the environment in which plants and animals live and the nutrients they draw from may also lead to epigenetic changes when they are altered.
CD Genomics provides epigenetic sequencing services based on NGS high-throughput sequencing foundation, which requires lower starting sample size, lower cost, shorter time, and can reduce a large number of unnecessary redundant genes, improving the efficiency of research in specific target regions. Different epigenetic modifications lead to different changes in living organisms. Our epigenetic sequencing service helps customers to study epigenetic modifications at the genome level, to explore the mechanism of dynamic network formation of life regulation, and to explore heritable changes in gene expression, regulation and traits with no change in nucleic acid sequence. The common epigenetic sequencing methods we provide based on NGS technology are whole genome hydroxymethylation sequencing, ChIP-seq, RIP-seq, and MeRIP-seq.
We have successfully performed RIP-seq on dozens of species including rice, Arabidopsis, pig, chicken, sheep and others.
We have completed MeRIP-seq on fish, cattle, pigs, chickens, rice, cabbage, pears and dozens of other species.
Genome-wide hydroxymethylation sequencing | RIP-seq | MeRIP-seq |
---|---|---|
Cell sample volume > 5×106 Tissue sample volume > 100 mg gDNA > 1 μg |
Cell sample volume > 5×108 Tissue sample volume > 500 mg RNA > 1 μg |
Cell sample volume > 5×106 Tissue sample volume > 20 mg RNA > 500 ng |
Samples were placed in 1.5 mL EP tubes, sealed with sealing film and transported on dry ice or ice packs to avoid repeated freezing and thawing. |
Fig 2. Epigenetic sequencing workflow
CD Genomics has successfully sequenced a wide range of plant and animal epigenetics, and we provide customised experimental protocols and integrated bioinformatics analysis services to assist our clients with the analysis of large volumes of NGS-based data according to their research needs. Our scientific protocol design, strict standardised service procedures and professional technical team are committed to providing you with the highest quality project services. If you are interested in us, please feel free to contact us.
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CD Genomics is propelling the future of agriculture by employing cutting-edge sequencing and genotyping technologies to predict and enhance multiple complex polygenic traits within breeding populations.