Next-generation sequencing (NGS) is the recommended approach to comprehensively study CRISPR editing. NGS-based targeted sequencing provides a cost-effective solution for confirming CRISPR-induced editing by focusing on targeted modification regions. CD Genomics, a company specializing in agricultural genomics services, is at the forefront of providing CRISPR sequencing services for agricultural research.

Introduction to CRISPR sequencing

Clusters of regularly spaced short palindromic repeats and CRISPR-associated protein 9 (CRISPR-Cas9) is a commonly used gene editing technology that has been applied to many biological challenges, including the genetic modification of agricultural crops. Some of the most pressing challenges in the areas of food security and environmental sustainability can be addressed through the use of this technology. CRISPR can be used to identify and characterize genes responsible for stress responses and diseases. Genetic modification using CRISPR can then enable plants to resist pests, diseases and other adversities, and improve crop quality. Interestingly, combining CRISPR/Cas9 with next-generation sequencing (NGS) has the potential to accelerate the identification, validation, and targeting of high-value targets, advancing sustainable agriculture.

Fig. 1. Application of the CRISPR/Cas9 system for imparting agronomically important traits in rice, wheat, and maize. (Ceasar et al., 2022)

Our CRISPR sequencing solution

CD Genomics provides reliable and affordable CRISPR sequencing solutions for breeders and agricultural researchers. We aim to accelerate your CRISPR agricultural breeding programs, including enhanced crop traits, higher yields, and increased resistance to pests, diseases, and environmental stresses, etc. Our NGS platform delivers high-resolution qualitative and quantitative information across the entire range of modifications, meeting any throughput requirement, and can be used to monitor off-target effects.

Our NGS can be used at all stages of the genome editing workflow, from analyzing CRISPR off-target effects by whole genome sequencing to confirming CRISPR knockdowns and other edits by targeted sequencing. Follow-up studies can then be performed using applications such as methylation analysis and gene expression profiling by RNA sequencing to assess the functional impact of a given gene edit. Our state-of-the-art laboratory facilities combined with a team of highly-skilled scientists and technicians allow us to provide accurate and reliable sequencing results with fast turnaround times.

What we offer

• CRISPR Screening Sequencing for Agriculture
• CRISPR Validation Sequencing for Agriculture
• CRISPR Off-target Sequencing for Agriculture

Support your CRISPR gene editing for agriculture with NGS

Our CRISPR sequencing service provides an efficient, quantifiable, and comprehensive way to measure the level of on-target and off-target editing. NGS offers significant advantages in analyzing CRISPR editing:

• NGS can be used to assess the sequence of an entire genome or a specific region of interest through targeted enrichment techniques such as hybridization capture and amplicon sequencing. 
• NGS is a method for comprehensively characterizing insertion/deletion (indel) features after CRISPR genome editing experiments.
• Amplicon sequencing using NGS can identify and quantify the frequency of insertion deletions resulting from non-homologous end joining (NHEJ) after the introduction of CRISPR-generated double-strand breaks (DSBs).
• With amplicon sequencing, you can also quantify the correct homology-directed repair (HDR) events and determine the percentage of target alleles that successfully undergo perfect HDR, thus defining a clear picture of editing events at the target locus.
• NGS allows multiple target and off-target loci to be studied in a single reaction.

Advantages of our services

• Cutting-edge technology

We utilize NGS to provide accurate and efficient gene editing solutions. Our laboratories are equipped with state-of-the-art equipment and utilize cutting-edge bioinformatics tools to analyze sequencing results.

• Team of experts

A team of experienced scientists, bioinformaticians, and technicians who are well-versed in CRISPR sequencing technology and agricultural genomics.

• Customized solutions

We offer customized CRISPR sequencing solutions, working closely with our clients to define project goals, and optimize experimental protocols.

• Quality assurance

We adhere to strict quality control measures throughout the CRISPR sequencing process. From sample preparation to data analysis, every step is carefully executed to ensure accurate and reliable results.

CD Genomics is proud to offer comprehensive CRISPR sequencing solutions for agricultural research. With our expertise in targeted gene editing, off-target analysis, and a wide range of applications, we help breeders and researchers be able to harness the power of CRISPR sequencing to advance sustainable agriculture. If you are interested, please feel free to contact us.

Reference

1. Ceasar, Antony. Genome-editing in millets: current knowledge and future perspectives. Molecular Biology Reports. 49.1 (2022): 773-781.